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FRANCE
CHITINE |
METHODS OF ANALYSES OF CHITOSAN |
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Appearance Particle size Loss on drying Loss on ignition pH Viscosity Degree of deacetylation Heavy metal Pesticides Bacteriology |
Gross observation JIS standard sieves 100 grs sample in oven at 110 °C for three hours 5gr in furnace at 900 °C for three hours 1% distill water solution Brook-field viscosimeter LVT model Infra red spectrophotometer Mineralisation and titrition by atomic absorption
Standard methods described in Guildelines for sea Food. Total Plate Count, Coliform, Staphyloccus aureus, Salmonelle sp., vibrio sp. |
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A |
Origine and grade |
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Squid bone |
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Shrimp shell cosmetic and pharmaceutical grade |
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Shrimp shell food grade |
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Shrimp shell technical grade |
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B |
Viscosity in CPS |
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(1gr of chitosan in 99gr acetic acid 1% solution at 25 °C) |
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Very high viscosity |
>4000 |
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High viscosity |
<2000>500 |
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Medium viscosity |
<500>100 |
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Low viscosity |
<100>20 |
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Very low viscosity |
<20>5 |
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Extra low viscosity |
<5 |
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Degree of deacetylation |
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for DDA lower than 80% |
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for DDA between 80 and 85% |
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for DDA between 85 and 90% |
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for DDA more than 90% |
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